Composite

Part:BBa_K5117046:Design

Designed by: Jenny Sauermann   Group: iGEM24_TU-Dresden   (2024-09-28)


PcotYZ-BsRBS-BhrPET-L3-CotY-B0014


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 695
    Illegal AgeI site found at 740
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

addition of the start codon of BhrPET,

addition of a rigid linker encoding the amino acids GGGEAAAKGGG,

addition of a 10 bp spacer downstream the CotY coding sequence

Source

Bacillus subtilis and Bacterium HR29